Chemistry, like all the natural sciences, begins with the direct observation of nature¡ª in this case, of matter.541-14-0, Name is (S)-3-Hydroxy-4-(trimethylammonio)butanoate, SMILES is O=C([O-])C[C@H](O)C[N+](C)(C)C, belongs to chiral-catalyst compound. In a document, author is Dou, Zhe, introduce the new discover, Application In Synthesis of (S)-3-Hydroxy-4-(trimethylammonio)butanoate.
A novel carboxylesterase AcEst1 was identified from Acinetobacter sp. JNU9335 with high efficiency in the biosynthesis of chiral precursor of Edoxaban through kinetic resolution of methyl 3-cyclohexene-1-carboxylate (CHCM). Sequence analysis revealed AcEst1 belongs to family IV of esterolytic enzymes and exhibits < 40% identities with known carboxylesterases. The optimum pH and temperature of recombinant AcEst1 are 8.0 and 40 degrees C. Substrate spectrum analysis indicated that AcEst1 prefers substrates with short acyl and alcohol groups. AcEst1 was highly active in the hydrolysis of CHCM with k(cat) of 1153 s(-1) and displayed high substrate tolerance. As much as 2.0 M (280 g.L-1) CHCM could be enantioselectively hydrolyzed into (S)-CHCM by merely 0.08 g.L-1 AcEst1 with ee(s) of > 99% (S) and substrate to catalyst ratio (S/C) of 3500 g.g(-1). These results indicate that the novel AcEst1 is a promising biocatalyst in the synthesis of chiral carboxylic acids.
The proportionality constant is the rate constant for the particular unimolecular reaction. the reaction rate is directly proportional to the concentration of the reactant. I hope my blog about 541-14-0 is helpful to your research. Application In Synthesis of (S)-3-Hydroxy-4-(trimethylammonio)butanoate.
Reference:
Chiral Catalysts,
,Chiral catalysts – SlideShare