Application In Synthesis of H-Leu-NH2.HCl. The reaction of aromatic heterocyclic molecules with protons is called protonation. Aromatic heterocycles are more basic than benzene due to the participation of heteroatoms. Compound: H-Leu-NH2.HCl, is researched, Molecular C6H15ClN2O, CAS is 10466-61-2, about Semisynthesis of human growth hormone-releasing factor by trypsin catalyzed coupling of leucine amide to a C-terminal acid precursor. Author is Bongers, Jacob; Offord, Robin E.; Felix, Arthur M.; Campbell, Robert M.; Heimer, Edgar P..
Human growth hormone-releasing factor, GRF(1-44)-NH2, was synthesized by trypsin catalyzed coupling of Leu-NH2 to Arg43 of the precursor, GRF(1-43)-OH, prepared by solid phase peptide synthesis. The semisynthetic GRF(1-44)-NH2 was fully characterized and showed full potency in the rat pituitary in vitro bioassay. Conversion to GRF(1-44)-NH2 was limited to 60-70% in both 75% v:v N,N-dimethylacetamide and 95% v:v 1,4-butanediol, due to competing transpeptidations at Arg41 and Arg38 generating [Leu42]-GRF(1-42)-NH2 and [Leu39]-GRF(1-39)-NH2 side products, resp. The rates of formation and yields of GRF(1-44)-NH2 vs. pH, Leu-NH2 concentration, and solvent composition were also studied.
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