The preparation of ester heterocycles mostly uses heteroatoms as nucleophilic sites, which are achieved by intramolecular substitution or addition reactions. Compound: H-Leu-NH2.HCl( cas:10466-61-2 ) is researched.Safety of H-Leu-NH2.HCl.Hill, Christopher R.; Tomalin, Geoffrey published the article 《A conductometric method for the assay of amidase and peptidase activities》 about this compound( cas:10466-61-2 ) in Analytical Biochemistry. Keywords: peptidase amidase determination conductometry; trypsin determination conductometry; chymotrypsin determination conductometry. Let’s learn more about this compound (cas:10466-61-2).
The enzyme-catalyzed hydrolysis of peptide and amide substrates results in the generation of ionic species, which in turn lead to a change in the elec. conductivity of the reaction medium. By recording this phys. property, it is possible to study the kinetics of the hydrolytic reactions. The principle of the conductometric method is given, and its application to the assay of proteolytic enzymes is illustrated by a number of reactions catalyzed by chymotrypsin Aα (EC 3.4.21.1) and trypsin (EC 3.4.21.4). The measured signal is related to product concentration by the molar conductivity coefficient (Λb). The measurement of Λb is described; it varies with the exptl. conditions, particularly when different buffer systems are used. The kinetic results (i.e., values of kcat and Km) for the chymotrypsin Aα and trypsin-catalyzed hydrolysis of a number of specific peptide and amide substrates are given. The results agree closely with previously published values obtained by a number of other assay techniques.
This compound(H-Leu-NH2.HCl)Safety of H-Leu-NH2.HCl was discussed at the molecular level, the effects of temperature and reaction time on the properties of the compound were discussed, and the optimum reaction conditions were selected.