The reaction of an aromatic heterocycle with a proton is called a protonation. One of articles about this theory is 《The specificity of leucine aminopeptidase》. Authors are Smith, Emil L.; Slonim, N. Balfour.The article about the compound:H-Leu-NH2.HClcas:10466-61-2,SMILESS:N[C@@H](CC(C)C)C(N)=O.[H]Cl).HPLC of Formula: 10466-61-2. Through the article, more information about this compound (cas:10466-61-2) is conveyed.
A highly purified preparation of leucine aminopeptidase (I) from hog intestinal mucosa, activated by Mn++ or Mg++, was found to hydrolyze only a single peptide bond of glycyl-L-leucinamide. The purification procedure of Smith and Bergmann (C.A. 38, 4965.7) was supplemented by precipitation with 33% acetone at room temperature followed by dialysis and removal of precipitate Since glycyl-L-leucine is not hydrolyzed by I, hydrolysis is thought to occur at the terminal peptide bond. I is devoid of endopeptidase activity. Compounds with free NH2 groups, such as L-leucyl-L-glutamic acid and L-glutamyl-L-leucinamide, are readily hydrolyzed, the latter compound only at one peptide bond. L-Leucylglycylglycine and L-leucylglycine are hydrolyzed most rapidly, whereas L-leucyl-L-glutamic acid is acted upon more slowly. Glycylglycyl-DL-leucylglycine is hydrolyzed very slowly. It is pointed out that in view of these findings the concept of the aminoexopeptidase must be amended to the extent that the free NH2 group need not be present on the amino acid residue which possesses the sensitive peptide bond. The synthesis of a number of aminopeptidase substrates is described. A simple synthesis for L-leucinamide-HCl by amidation of L-leucine methyl ester-HCl is reported. Glycyl-L-leucinamide-HCl ([α]26D = -19.0° (5% solution in water)) was synthesized from carbobenzoxyglycyl-L-leucine methyl ester. Syntheses for L-glutamyl-L-leucinamide ([α]27D = +6.7° (2.1% in water)) from carbobenzoxy-L-glutamyl anhydride and L-leucine methyl ester and for L-leucyl-L-glutamic acid ([α]24D = +10.5° (2% in M HCl)) from carbobenzoxy-L-leucine hydrazide are reported.
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