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Functional dihydro-1H-imidazole derivatives for MALDI signal enhancement of a lysine-specific chemical modification
The enhancement of signal sensitivity and quantification of low-abundance proteins is of great importance in proteomic analysis. The preparation of 2-methoxy-4,5-dihydro-1H-imidazole (MeO-DHIM) derivatives was accomplished by one-step synthesis of O-methyl-isourea. The signal enhancement induced by these attached moieties increases in an order of compound 1 ? 4 < 2 < 5 ? 6 < 3 in MALDI mass spectrometry. Peptide-compound 3 adduct was approximately 20 times signal enhancement of the unmodified peptide and of 9 times of the peptide-compound 1 adduct. This result demonstrates that the rational designed organic molecules are capable of providing a sensitive tool in the detection of low-abundance proteins in proteomics. If you are hungry for even more, make sure to check my other article about 21436-03-3. Related Products of 21436-03-3
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Chiral Catalysts,
Chiral catalysts – SlideShare